There are five designated aid stations on the ride, servicing participants with water, toilets and first aid at the following locations:
- 20 km G. J. Hoskins Reserve Altona North
- 40 km Keilor Village
- 50 km Caroline Springs
- 80 km Werribee
These medical stations will be serviced by mobile first aid units at each of the aid stations. These trailers provide shelter for treatment and are stocked appropriately with first aid medical supplies.
There will also be a first aid vehicle travelling at the rear of the ride with paramedics on board.